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. 2019 Jul 19;20(14):3541. doi: 10.3390/ijms20143541

Figure 1.

Figure 1

CD271 receptor expression in squamous cell carcimoma in vitro. (A) Left panel, CD271 expression in normal human keratinocytes (NK), keratinocytes from primary squamous cell carcinoma (SCC), and SCC15 cell line (SCC15) by Western blotting. Right panel, CD271/β-actin RQ (relative quantification) by densitometry analysis performed by ImageJ software. (B) Percentage of CD271 positive cells in SCC12B, SCC13, and SCC15 cell lines evaluated by FACS analysis. (C) Total area (or spheroid size) of SCC cell line derived spheroids, evaluated by ImageJ software analysis at 24 h after SCC cell plating. (D) Upper panel: representative images of SCC12B, SCC13, or SCC15 derived spheroids. Scale bar = 100 μm. Lower panel: SCC12B, SCC13, or SCC15 image masks generated from ImageJ software analysis and used to calculate the total area/size of each spheroid. For all results, data represent the mean from three independent experiments ± SEM. * for p < 0.05, ** for p < 0.01.