Figure 3.

Identification of two main patient subsets based on proteomic differences of AML cells with high and low constitutive release. Eight of the 16 patients included in the proteomic studies belonged to the cluster characterized by generally high constitutive mediator release and the eight others showed low/intermediate secretion (Figure 2); 256 proteins differed significantly between these two groups. We performed an unsupervised hierarchical cluster analyses (Euclidean measure, and complete distance) based on the levels of these proteins, and the left part demonstrates the dendrogram and heat map; blue indicates low protein levels and green high levels. Two main clusters were then identified corresponding to the high and low/intermediate secretion patients except for one outlier patient (left column, red color indicating high release). As expected, the two main clusters were heterogeneous with regard to mutational frequencies (middle panel) and did not differ with regard to clinical or biological characteristics either (right panel).