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. 2019 Jul 13;10(7):532. doi: 10.3390/genes10070532

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The mutation in mt-Nd2 is associated with alteration of tryptophan levels. (A) A ratio of NAD⁺ to NADH was measured in liver tissues of young (3 months old) female B6-mt^BPL and B6-mt^ALR mice. No difference was observed (descriptive p = 0.3737, t-test). n = 9/strain. BPL: B6-mt^BPL; ALR: B6-mt^ALR. (B) A region of the NMR spectra of a well-separated signal of tryptophan in B6-mt^ALR fibroblast cell line (red) and B6-mt^BPL fibroblast cell line (black) is displayed. n = 5 (B6-mt^ALR cell lines), n = 7 (B6-mt^BPL cell lines). (C) Tryptophan levels identified in panel B were quantified and compared between the groups. The mean value of the two signals was compared. Descriptive p = 0.0295, t-test.