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. 2019 Jul 15;8(7):725. doi: 10.3390/cells8070725

Figure 5.

Figure 5

Over-expression of PEX11B increases the expression of MAVS protein. U251 cells were transduced with lentiviruses encoding AcGFP alone or AcGFP plus myc-tagged PEX11B for 48 h and then transfected with poly(I:C) (+) or an empty plasmid vector (−) for 12 h. The cell lysates were processed for immunoblot analyses with a mouse monoclonal antibody to MAVS, rabbit polyclonal PEX13, goat polyclonal antibody to GFP, and a mouse monoclonal antibody to the myc epitope. The relative levels of MAVS and PEX13 (compared to actin) from three independent experiments were averaged and plotted. Bars represent standard error of the mean. ** p < 0.01, * p < 0.05.