Table 2.
Primers used for qPCR.
| Gene | Primers | Sequences 5′ → 3′ |
|---|---|---|
| β-Actin | ACT-F ACT-R |
TCATGAAGTGTGACGTTGACA CCTAGAAGCATTTGCGGTGCAC |
| HLA-DQA1*01 | DQA1*01-F DQA1*R |
CGGTGGCCTGAGTTCAGCAA GGAGACTTGGAAAACACTGTGACC |
| HLA-DQA1*02 | DQA1*02-F DQA1*R |
AAGTTGCCTCTGTTCCACAGAC GGAGACTTGGAAAACACTGTGACC |
| HLA-DQA1*03 | DQA1*03-F DQA1*R |
CTCTGTTCCGCAGATTTAGAAGA GGAGACTTGGAAAACACTGTGACC |
| HLA-DQA1*05 | DQA1*05-F DQA1*R |
CTCTGTTCCGCAGATTTAGAAGA GGAGACTTGGAAAACACTGTGACC |
| HLA-DQB1*02 | DQB1*02-F DQB1*R | TCTTGTGAGCAGAAGCATCT CAGGATCTGGAAGGTCCAGT |
| HLA-DQB1*03 | DQB1*03-F DQB1*R | CGGAGTTGGACACGGTGTGC CAGGATCTGGAAGGTCCAGT |
| HLA-DQB1*05 | DQB1*05 DQB1*R |
ACAACTACGAGGTGGCGTACC CAGGATCTGGAAGGTCCAGT |
| HLA-DQB1*06 | DQB1*06-F DQB1*R |
CAGATCAAAGTCCGGTGGTTTC CAGGATCTGGAAGGTCCAGT |