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. 2019 Jul 2;11(7):927. doi: 10.3390/cancers11070927

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoreactive score of the phosphatase and tensin homologues deleted on chromosome 10 (PTEN). Immunostaining reveals that PTEN shows no difference between control and AURKA transgenic zebrafish. (A) Representative images of immunohistochemistry (IHC) staining for PTEN in control, AURKA(WT), and AURKA(V352I) at 3, 5, 7, 9, and 11 months; (B) Selected enlarged images show the PTEN signals from AURKA(WT)—7 M and AURKA(V352I)—7 M; (C) Statistical analysis of PTEN immunostaining IRS score at 3, 5, 7, 9 and 11 months. IRS score of control fish (Tg(fabp10a:EGFP-mCherry) abbreviated as mC, AURKA(WT) and AURKA(V352I) is shown in red (3 M), orange (5 M), green (7 M), purple (9 M), and blue (11 M). Statistical analysis of results was performed using a two-tailed Student’s t-test. The error bar means standard deviation.