Table 1.
Primer sequence of qPCR for human AURKA, zebrafish actin, lipogenic factors (pparγ, srebp1, chrebp), lipogenic enzymes (dgat2, pap, fasn), fibrosis markers (col1a1, ctgfa, hpse) and cell cycle related genes (ccne1, cdk1, cdk2).
| Gene Name | Primer Name | Sequence (5′ to 3′) |
|---|---|---|
| AURKA | Q-AURKA-F | TGGAATATGCACCACTTGGA |
| Q-AURKA-R | ACTGACCACCCAAAATCTGC | |
| actin | Q-actin-F | CTCCATCATGAAGTGCGACGT |
| Q-actin-R | CAGACGGAGTATTTGCGCTCA | |
| pparγ | Q-pparγ- | GGTTTCATTACGGCGTTCAC |
| Q-pparγ-R | TGGTTCACGTCACTGGAGAA | |
| srebp1 | Q-srebp1-F | CATCCACATGGCTCTGAGTG |
| Q-srebp1-R | CTCATCCACAAAGAAGCGGT | |
| chrebp | Q-chrebp-F-2 | GGAGATGGACTCGCTCTTTG |
| Q-chrebp-R-2 | GCAGAGGCTCAAAAGTGTCC | |
| dgat2 | Q-dgat2-F | TGGGGCTTTTTGTAACTTCG |
| Q-dgat2-R | TCTTCCTGGTGCACAGTCC | |
| pap | Q-pap-F | CAGTTCTTCCTGATTGCTGC |
| Q-pap-R | TCCTCAAAGCTTAGTTCGGG | |
| fasn | Q-fasn-F | ATCTGTTCCTGTTCGATGGC |
| Q-fasn-R | AGCATATCTCGGCTGACGTT | |
| col1a1 | Q-col1a1-F | TATTGGTGGTCAGCGTGGTA |
| Q-col1a1-R | TCCTGGAGTACCCTCACGAC | |
| ctgfa | Q-ctgfa-F | TGTGTGTTTGGTGGAATGGT |
| Q-ctgfa-R | GGAGTCACACACCCACTCCT | |
| hpse | Q-hpse-F | GCTCTGGTTTGGAGCTCATC |
| Q-hpse-R | GAAATCCCGACCAAGTTGAA | |
| ccne1 | Q-ccne1-F | TCCCGACACAGGTTACACAA |
| Q-ccne1-R | TTGTCTTTTCCGAGCAGGTT | |
| cdk1 | Q-cdk1-F | CTCTGGGGACCCCTAACAAT |
| Q-cdk1-R | CGGATGTGTCATTGCTTGTC | |
| cdk2 | Q-cdk2-F | CAGCTCTTCCGGATATTTCG |
| Q-cdk2-R | CCGAGATCCTCTTGTTTGGA |