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. 2019 Jul 18;8(7):739. doi: 10.3390/cells8070739

Figure 1.

Figure 1

Cytotoxicity of Ala and its effect on LPS-activated BV2 cell proliferation. (A) Cytotoxicity of Ala for BV2 cells after 24 h. (B) Influence of Ala on the morphological changes of LPS-activated BV2 cells after 24 h. The arrow indicates the ameboid change in BV2 cells. (C) BV2 cell proliferation was assessed by 5-ethynyl-2’-deoxyuridine (EdU) (green) and 4′,6-diamidino-2-phenylindole (DAPI, blue) staining. (D) The inhibitory effect of Ala on LPS-activated BV2 cell cycle progression by propidium iodide (PI) staining. The concentration of LPS in (B–D) is 100 ng/mL. The concentration of Ala in (B–D) is 2 μM. (n = 3) (* p < 0.05) (** p < 0.01) (*** p < 0.001).