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Effect of Ala on interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-activated BV2 cells: (A) mRNA detection of (1) IL-1β, (2) IL-6, and (3) TNF-α (the BV2 cells were pretreated with Ala for 30 min and stimulated with LPS for another 6 h); (B) mRNA expression of (1) IL-1β, (2) IL-6, and (3) TNF-α at 6 and 24 h; (C) translation level detection of (1) IL-1β, (2) IL-6, and (3) TNF-α at 24 h by ELISA. (n = 3) (* p < 0.05) (** p < 0.01) (*** p < 0.001).
