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. 2019 Jul 18;8(7):739. doi: 10.3390/cells8070739

Figure 5.

Figure 5

(A) Effect of Ala on PC12 cell viability after 24 h. (B) Ratio of PC12 cell apoptosis (early apoptosis + late apoptosis) and necrosis in a coculture system with BV2 cells and PC12 cells. (C) PC12 cell apoptosis and necrosis detection in the coculture system with BV2 cells and PC12 cells by flow cytometry and immunofluorescence. PC12 cells were cocultured with LPS-stimulated BV2 cells with or without pretreatment with 2 μM of Ala for 24 h. The PC12 + LPS group without BV2 (PC12 + LPS) was designed separately to exclude the direct effect of LPS on PC12 cells. The apoptotic cells were the annexin V-positive cells, the necrotic cells were the annexin V-negative and PI-positive cells, and the normal cells were the annexin V-negative and PI-negative cells. The green fluorescence represents annexin V-positive cells, and the red fluorescence represents PI-positive cells. The concentration of LPS in (B,C) is 100 ng/mL. (n = 3) (* p < 0.05) (** p < 0.01) (*** p < 0.001).