Figure 2.

Knockdown of endogenous GR exacerbates mild HI injury in the male neonatal rat pup brain. GR siRNA (100 pmol) or negative control (100 pmol) was injected by ICV injection 48 h on postnatal day 7 (P7) before HI-injury. HI-injury was performed on postnatal day 9 (P9) for 60 min in 8% FiO₂. We separated sex into the following groups: negative control (negative control: male, n = 5; female, n = 8) versus GR siRNA (male, n = 5; female, n = 11). In the male population, GR siRNA increased infarct volume. No significant difference was seen in the female population, although the total brain injury was increased with a mild hypoxic-ischemic model. Data are means ± SEM, * p = 0.0315, GR siRNA vs. negative control.