Figure 4.

Repression of GR worsens short-term neurobehavioral deficits in the neonatal male rat with mild HI injury. The neonatal male rats received GR siRNA (100 pmol) or negative control (100 pmol) by ICV injection 48 h before HI-injury (8% O₂; 60 min). The sham group did not undergo HI-injury treatment. The short-term neurobehavioral assessment was taken two, four, and six days after HI insult. (A) Mortality rate with Groups: negative control without injury (sham); (n = 12) versus negative control with HI injury (n = 13) versus GR siRNA (n = 14) with HI injury. Subsequent weight and behavioral studies do not include animals that did not survive. (B) Weight added since HI injury, (C) geotaxis test, (D) righting reflex, and the (E) wire hanging test were evaluated accordingly. Groups for weight and short-term behavior were negative control without injury (sham); (n = 12) versus negative control with HI injury (n = 13) versus GR siRNA (n = 11) with HI injury. Data are means ± SEM, * p < 0.05, *** p < 0.0001.