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. 2019 Jul 22;20(14):3583. doi: 10.3390/ijms20143583

Figure 2.

Figure 2

Cytokine and chemokine expression in HBMEC co-stimulated with Ureaplasma spp. and LPS. Expression of mRNA and protein secretion were determined using qRT-PCR (a, d, g, j, m), RNA sequencing (b, e, h, k, n), and multi-analyte immunoassay (c, f, i, l, o). Intracellular IL-8 was additionally quantified by flow cytometry (p). Data are presented as means ± SD and were obtained from n ≥ 3 individual experiments (# p < 0.05, ## p < 0.01 vs. LPS; § p < 0.05, §§ p < 0.01, §§§ p < 0.001 vs. broth+LPS). CXCL: C-X-C chemokine ligand; HBMEC: human brain microvascular endothelial cells; IL: interleukin; LPS: lipopolysaccharide; MCP: monocyte chemoattractant protein; RPKM: reads per kilo base per million mapped reads; SD: standard deviation; Up3: Ureaplasma parvum serovar 3; Uu8: Ureaplasma urealyticum serovar 8.