Table 1.
Proteins corresponding to validated mRNA sequences (RPKM > 100) found in Paracoccidioides EVs. The sequences are grouped according with their functions. Coverage used for validation is indicated, as well as the gene access codes for each isolate.
| Pb18 Feature ID | Pb3 Feature ID | Pb01 Feature ID | Sequence Description | Coverage | GO |
|---|---|---|---|---|---|
| Protein Modification | |||||
| PAAG_08003 | hsp70-like protein | 100% | ion binding | ||
| PAAG_05980 | ubiquitin-conjugating enzyme e2-16 kda | 80% | ligase activity | ||
| PADG_07715 | hsp90-like protein | 70% | protein folding, response to stress | ||
| PADG_01605 | PABG_03078 | PAAG_07080 | polyubiquitin | 70% | protein modification process |
| PADG_11111 | nuclear transport factor 2 | 75% | protein targeting | ||
| PAAG_05679 | ATP-dependent molecular chaperone hsc82 | 90% | nucleic acid binding transcription factor activity, protein folding | ||
| Carbohydrate Metabolism | |||||
| PADG_02145 | glycogen phosphorylase | 70% | carbohydrate metabolic process | ||
| Translation | |||||
| PADG_05025 | 60s ribosomal protein l26 | 70% | translation | ||
| PADG_00692 | PAAG_11418 | elongation factor 1-alpha | 100% | translation | |
| PADG_03326 | 40s ribosomal protein s9 | 80% | translation | ||
| PABG_05744 | pre-mrna splicing factor | 70% | translation | ||
| Oxidation-Reduction | |||||
| PAAG_03216 | thiol-specific antioxidant | 90% | oxidoreductase activity | ||
| Transport | |||||
| PAAG_11262 | hsp7-like protein | 100% + MR | transmembrane transport | ||
| PAAG_03058 | high-affinity methionine permease | 100% + 5′R | transmembrane transport | ||
| PAAG_07634 | gtp-binding protein rhoa | 70% | vesicle-mediated transport | ||
| DNA Metabolism or Biogenesis | |||||
| PADG_00873 | PABG_02444 | PAAG_07099 | histone h3 | 100% | chromosome organization |
| PABG_05588 | PAAG_08917 | histone h2a | 90% | chromosome organization | |
| PAAG_08918 | histone h2b | 70% | chromosome organization | ||
| PADG_06568 | tctp family protein | 50% | cell differentiation | ||
| PABG_03449 | PAAG_08247 | calmodulin | 70% | ion binding | |
| Other/Unknown Function | |||||
| PADG_02280 | hypothetical protein | 75% | Unknown | ||
| PADG_02399 | calcium-binding protein | 70% | Unknown | ||
| PADG_04049 | hypothetical protein | 60% | Unknown | ||
| PADG_08402 | hypothetical protein | 100% | Unknown | ||
| PADG_12385 | ser thr protein phosphatase family protein | 60% | Unknown | ||
| PAAG_00340 | conserved hypothetical protein | 85% | unknown | ||
| PAAG_12435 | hypothetical protein | 80% | unknown | ||
| PAAG_12692 | ATP synthase subunit beta | 95% reverse | unknown | ||
| PAAG_12694 | plant senescence-associated protein | 100% reverse | unknown | ||
| PAAG_02087 | kelch-like protein 38 | 70% | unknown | ||