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. 2019 Jul 19;20(14):3530. doi: 10.3390/ijms20143530

Figure 2.

Figure 2

Evaluation of enzyme inactivation methods. Reaction mixtures (2 mM GDP-Man, 0.01 mg/mL MfGM35E, pH 8) were incubated at 37 °C. At defined time points, samples were 10× diluted in various inactivation solutions (0.01 M NaOH, 45% acetonitrile (ACN), acetonitrile/methanol (MeOH) (50:50), or 100% ethanol (EtOH)). The y-axis represents the conversion of GDP-Man towards GDP-L-Gal.