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. 2019 Jul 19;20(14):3530. doi: 10.3390/ijms20143530

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sequence and structural analysis of MfGM35E. (a) Multiple sequence alignment of the GM35Es from Methylacidiphilum fumariolicum (Mf), Arabidopsis thaliana (At), Oryza sativa subsp. japonica (Os_j), and Oryza sativa subsp. indica (Os_i). Yellow, GxxGxxG motif; blue, YxxxK motif and upstream serine (S); green, catalytic cysteine (C) and lysine (K); purple, conserved arginine (R). (b) Representation of the active site of MfGM35E (blue) and AtGM35E (yellow). The catalytic C and K are highlighted, as well as the conserved R. GDP-mannose is colored by element (green).