Figure 3.

Arachidonic acid induces endogenous Ca²⁺ release and extracellular Ca²⁺ influx in hCMEC/D3 cells. (A) 30 μM AA evoked endogenous Ca²⁺ release under absence of extracellular Ca²⁺ (0Ca²⁺) conditions followed by a second increase in [Ca²⁺]_i upon Ca²⁺ restitution to the bath in the continuous presence of the agonist. (B) 50 μM AA evoked endogenous Ca²⁺ release under 0Ca²⁺ conditions followed by a second increase in [Ca²⁺]_i upon Ca²⁺ restitution to the bath in the continuous presence of the agonist. (C) Mean ± SE of the amplitude of endogenous Ca²⁺ release and extracellular Ca²⁺ influx induced by each concentration of AA. The asterisk indicates p < 0.05.