Figure 6.

TRPV4 mediates arachidonic acid-induced extracellular Ca²⁺ influx in hCMEC/D3 cells. (A) Intracellular Ca²⁺ response to AA (30 μM) recorded in the presence and in the absence of RN-1734 (20 μM, 20 min). (B) Mean ± SE of the amplitude of AA-evoked Ca²⁺ influx in the absence and in the presence of RN-1734 (RN). The asterisk indicates p < 0.05. (C) AA (30 μM) evoked an increase in the [Ca²⁺]_i in the presence of extracellular Ca²⁺ upon 30 min treatment with CPA (10 μM) to deplete the ER Ca²⁺ store and fully activate store-operated Ca²⁺ influx (SOCE). AA-evoked Ca²⁺ influx was abolished by pretreating the cells with RN-1734 (20 μM, 20 min). (D) Mean ± SE of the amplitude of AA-induced intracellular Ca²⁺ signals in the absence and in the presence of RN-1734 (RN) upon SOCE activation with CPA (10 μM). The asterisk indicates p < 0.05.