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. 2019 Jul 9;8(7):689. doi: 10.3390/cells8070689

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Arachidonic acid induces NO release in a Ca²⁺-dependent manner in hCMEC/D3 cells. (A) AA (20 μM) evoked a robust increase in the fluorescence in hCMEC/D3 cells loaded with the NO-selective fluorophore, 4-amino-5-methylamino-2’,7’-difluorofluorescein (DAF-FM). AA-elicited NO release was abrogated by L-N^G-Nitro-L-arginine methyl ester (L-NAME; 100 μM, 1 h) or BAPTA (30 μM, 2 h). (B) Mean ± SE of the amplitude of AA-elicited NO release under the designated treatments. For L-NAME and BAPTA, the number of total cells analysed is indicated in the brackets. The asterisk indicates p < 0.05. (C) AA (30 μM) and ETYA (30 μM) induced an increase in DAF-FM fluorescence with overlapping kinetics and amplitude. (D) Mean ± SE of the amplitude of AA- and ETYA-evoked NO release in hCMEC/D3 cells. NR means no response.