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. 2019 Jul 9;8(7):689. doi: 10.3390/cells8070689

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TPC1-2 and TRPV4 support arachidonic acid-induced NO production in hCMEC/D3 cells. (A) AA (30 μM) evoked robust NO release under control conditions in hCMEC/D3 cells. (B) AA (30 μM) induced NO release under 0Ca²⁺ conditions, although this signal was lower as compared to control conditions. However, readdition of Ca²⁺ to the perfusate caused a second increase in DAF-FM fluorescence. (C) RN-1734 (20 μM, 20 min) reduced AA-evoked NO release. AA was administered at 30 μM. (D) 2-APB (50 μM, 30 min) did not affect AA-induced NO production. AA was administered at 30 μM. (E) CPA (10 μM, 30 min) did not impair AA-evoked NO release. AA was administered at 30 μM. (F) GPN (200 μM, 30 min) remarkably reduced AA-induced NO production. AA was administered at 30 μM. (G) NED-19 (100 μM, 30 min) impaired AA-evoked NO release. AA was administered at 30 μM. (H) Mean ± SE of the amplitude of AA-evoked NO release under the designated treatments. The asterisk indicates p < 0.05. The total number of analysed cells is indicated in the brackets.