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. 2019 Jul 21;8(7):759. doi: 10.3390/cells8070759

Figure 2.

Figure 2

Cdc42 effects on actin dynamics (continued). (A) Myc-Cdc42/G12V, Myc-Cdc42/D118N, Myc-Cdc42/Q61LF37A, Myc-Cdc42/Q61L/Y40C, Myc-Cdc42/Q61LΔins, and Myc-Cdc42/Q61LT35A were exogenously expressed in BJ/hTERT SV40T cells. Myc-tagged Cdc42 was detected with a rabbit anti-Myc antibody, followed by an Alexa Fluor 488-conjugated donkey anti-rabbit antibody. Filamentous actin was visualized using TRITC-conjugated phalloidin. Arrow-heads mark transfected cells. The boxed areas are enlarged at the right-hand-side of the corresponding image. Scale bar, 20 µm. (B,C) Quantification of formation of filopodia and broad lamellipodia (B), and of actin filament organization (C). At least 100 transfected cells were scored for each aspect (as indicated) from three independent experiments. Data are means ± standard deviation.