Figure 4.

Rac1 effects on actin dynamics. (A) Myc-tagged wt and mutant Rac1 were exogenously expressed in BJ/hTERTSV40T cells. Myc-tagged proteins were detected with a rabbit anti-Myc antibody followed by an Alexa Fluor 488-conjugated donkey anti-rabbit antibody. Filamentous actin was visualized using TRITC-conjugated phalloidin. Arrow-heads mark transfected cells. The boxed areas are enlarged at the right-hand-side of the corresponding image. Scale bar, 20 µm. (B,C) Quantification of formation of filopodia and broad lamellipodia (B), and of actin filament organization (C). At least 100 transfected cells were scored for each phenotype (as indicated) from three independent experiments. Data are means ±standard deviation.