Table 2.
Components of the specialized remodeling and ER export machinery of GPI-APs in yeast and mammals.
| Process | Enzymes | |
|---|---|---|
| Yeast | Mammals | |
| Lipid remodeling of the GPI anchor | ||
| Inositol deacylation | Bst1 | PGAP1 |
| sn2 deacylation | Per1 | PGAP3 |
| sn2 reacylation with long saturated fatty acid | Gup1 | PGAP2 |
| Replacement of C26:0 diacylglycerol with C26:0 ceramide | Cwh43 | |
| Glycan remodeling of the GPI anchor | ||
| Removal of EtNP from Man2 | Ted1 | PGAP5 |
| Removal of EtNP from Man1 | Cdc1 | |
| COPII coat recruitment | ||
| p24 complex: Tretramers (αβγδ) or Heterodimers (2β2δ) α-members: Erp1, Erp5 and Erp6 β-member: Emp24 γ-members: Erp2, Erp3 and Erp4 δ-member: Erv25 |
p24 complex: Tretramers (αβγδ) or Heterodimers (2β2δ) [24] α-members: p24α1, p24α2 and p24α3 β-member: p24β1 γ-members: p24γ1, p24γ2, p24γ3, p24γ4 and p24γ5 δ-member: p24δ1 |
|
| COPII vesicle formation | ||
| COPII coat inner layer | Lst1/Sec23 | Sec24C-D/Sec23 |
| COPII coat outer layer | Sec13/Sec31 | Sec13/Sec31 |