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. 2019 Jul 11;11(7):972. doi: 10.3390/cancers11070972

Figure 6.

Figure 6

KIT inhibition attenuates MDA-P selection toward CSC induced by the double treatment with RH1. (A). Relative expression levels of KIT receptor and its ligand KITLG in MDA-P and MDA-R cells measured by RT-qPCR. The results are the mean of 3 independent experiments; bars are ± SD (ANOVA; * p < 0.05; ** p < 0.01). (B). Experimental time chart of cells short-time selection without and with KIT inhibition. (C). MDA-P (blue) or MDA-R (red) cells were pulse exposed or not exposed twice to 20 nM RH1 with or without of continuous treatment with KIT receptor inhibitor masitinib (5 µm). After 13 days cells were stained with CD44-FITC antibody and analyzed by flow cytometry. (D). MDA-P and MDA-R cells were subjected to sphere forming assay with or without 5µM masitinib treatment. Spheres were stained with MTT dye and counted using ImageJ software. The results are the mean of 3 independent experiments, two replicates each; bars are ± SD (ANOVA; * p <0.05; ** p < 0.01).