Skip to main content
. 2019 Jul 19;20(14):3548. doi: 10.3390/ijms20143548

Figure 5.

Figure 5

Ruxolitinib can hamper the increase of intracellular IFNγ and p-STAT3-S727 induced by MM soluble factors in HDNs. (A) Intracellular interferon-gamma levels, as detected by flow cytometry, in healthy, MGUS, and MM HDN cells are shown, with a representative flow cytometry image for each disease status. (B) Intracellular p-STAT3-S7272 levels, as detected by flow cytometry, in healthy, MGUS, and MM HDN cells are shown, with a representative flow cytometry image for each disease status. HDN isolated from healthy subjects were cultured in vitro or 3 h in the presence of conditioned media obtained from MM1.s or U266 cell lines with or without pan-JAK inhibitor 500 nM Ruxolitinib to assess autophagy, detected as the percentage of AVOs after treating with (C) and changes in phosphorylation of STAT3 on S727 (D). *** p < 0.0001; ** p < 0.001; * p < 0.05 (Student’s t-test).