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. 2019 Jul 15;11(7):989. doi: 10.3390/cancers11070989

Figure 2.

Figure 2

Suppression of p21 affects chromosome segregation in BeWo cells. (A) Western blot control for small interfering RNA (siRNA) transfection efficiency. BeWo cells were treated with scrambled siRNA (sicon) or siRNA against the untranslated region (UTR) of p21 (sip21 #1) or mixed siRNAs against the coding region of p21 (sip21 #2) for 48 h. (B) Quantification of defects in chromosome congression in metaphase cells treated as in (A) (n = 3, 100 metaphase cells per experiment and condition). The results are presented as mean ± standard error of the mean (SEM) and statistically analyzed. (C) Quantification of defects in chromosome segregation in anaphase cells treated as in (A) (n = 3, 100 anaphase cells per experiment and condition). The results are presented as mean ± SEM and statistically analyzed. ** p < 0.01; *** p < 0.001. (D) Representative images of confocal laser scanning microscopy are shown. Cells were stained for tubulin, pericentrin, anti-centromere antibody (ACA), and DNA. Scale bar: 7.5 µm. Arrow: Indicating either chromosome congression/segregation defect (4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining) or failure of centrosome integrity (pericentrin staining).