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. 2019 Jul 15;11(7):989. doi: 10.3390/cancers11070989

Figure 3.

Figure 3

p21 is required for cell motility. Single-cell tracking using time-lapse microscopy was performed with treated HTR cells. (A–C) Representative trajectories of individual cells (n = 30) treated with sicon (A), sip21 #1 (B), or sip21 #2 (C) are shown. (D) Control Western blot analysis shows the efficient knockdown of endogenous p21 in HTR cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control. (E–G) Accumulated distance (E), velocity (F), and directionality (G) of these cells were analyzed and statistically evaluated from three independent experiments. The results are shown as scatter plots. *** p < 0.001. (H–J) Representative trajectories are shown for individual HeLa cells (n = 30) with sicon (H), sip21 #1 (I), and sip21 #2 (J). (K) Control Western blot analysis of HeLa cells. GAPDH served as loading control. (L–N) Accumulated distance (L), velocity (M), and directionality (N) were evaluated. The data are derived from three independent experiments and shown as scatter plots. * p < 0.05, ** p < 0.01, *** p < 0.001.