Table 2.
Primer sequences for quantitative real-time polymerase chain reaction.
| Gene * | Primer Sequence (5′–3′) | Size (bp) | Accession No. |
|---|---|---|---|
| TLR4 | Forward: TCAGTTCTCACCTTCCTCCTG | 166 | NM_001113039.2 |
| Reverse: GTTCATTCCTCACCCAGTCTTC | |||
| MyD88 | Forward: GATGGTAGCGGTTGTCTCTGAT | 148 | NM_001099923.1 |
| Reverse: GATGCTGGGGAACTCTTTCTTC | |||
| IRAK1 | Forward: CAAGGCAGGTCAGGTTTCGT | 115 | XM_003135490.4 |
| Reverse: TTCGTGGGGCGTGTAGTGT | |||
| TRAF6 | Forward: CAAGAGAATACCCAGTCGCACA | 122 | NM_001105286.1 |
| Reverse: ATCCGAGACAAAGGGGAAGAA | |||
| Tollip | Forward: GCAGCAGCAACAGCAGAT | 133 | NM_001315800.1 |
| Reverse: GGTCACGCCGTAGTTCTTC | |||
| SIGIRR | Forward: ACCTTCACCTGCTCCATCCA | 205 | NM_001315689.1 |
| Reverse: TTCCGTCATTCATCTCCACCTC | |||
| Bcl-2 | Forward: AGCATGCGGCCTCTATTTGA | 120 | XM_021099593.1 |
| Reverse: GGCCCGTGGACTTCACTTAT | |||
| Bax | Forward: CTGACGGCAACTTCAACTGG | 200 | XM_003127290.5 |
| Reverse: CGTCCCAAAGTAGGAGAGGA | |||
| Fas | Forward: TGATGCCCAAGTGACTGACC | 103 | NM_213839.1 |
| Reverse: GCAGAATTGACCCTCACGAT | |||
| TNFR1 | Forward: CTGGCATTCTTCCTCTTCGTTG | 109 | NM_213969.1 |
| Reverse: CCGGCTCTCCCTCCTTTACA | |||
| TRADD | Forward: AGGCGTGCTTGGAGGCT | 124 | XM_021094047.1 |
| Reverse: GCGAAGATGAAATTCAAACAGC | |||
| FADD | Forward: CTGCGACAACGTGGGGA | 101 | NM_001031797.1 |
| Reverse: TCAGGTTTCGGGGATACTTC | |||
| GAPDH | Forward: ATGGTGAAGGTCGGAGTGAAC | 235 | NM_001206359.1 |
| Reverse: CTCGCTCCTGGAAGATGGT |
* TLR4, Toll-like receptor 4; MyD88, myeloid differentiation factor 88; IRAK1, interleukin-1 receptor-associated kinase 1; TRAF6, tumour necrosis factor receptor-associated factor 6; Tollip, toll-interacting protein; SIGIRR, single immunoglobulin interleukin-1 receptor-related molecule; Bcl-2, B-cell lymphoma-2; Bax, B-cell lymphoma-2-associated X protein; TNFR1, tumour necrosis factor receptor 1; TRADD, tumour necrosis factor receptor-associated death domain; FADD, Fas-associated death domain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.