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. 2019 Jul 10;20(14):3382. doi: 10.3390/ijms20143382

Figure 6.

Figure 6

Functional testing of E1/R4, E2/R5, and D1/R5, or D1/R6 interactions by cysteine mutagenesis. Representative current traces (A) and G/V curves (B) for the indicated channels. Current scale: 500 pA; time scale: 200 ms. Representative traces (C) and G/V curves (D) in CHO cells expressing Kv7.2 E1C/R4C channels in control solution or upon application of 1 mM dithiothreitol (DTT), as indicated by arrows. (E) Time-course of the effects of acute perfusion of DTT (1 mM), followed by H2O2 (0.5 mM) on instantaneously activated (IINST) or time-dependent (ISS) currents, expressed by Kv7.2 E1C/R4C channels. (F) Quantification of the effects of DTT (1 mM) and H2O2 (0.5 mM) on cells expressing the indicated channels. The asterisk indicates a value significantly different from that measured in control solution. (G) V½s of indicated channels in the absence (black bars) or in the presence (white bars) of 1 mM DTT, as indicated. Asterisks indicate significantly different values (p < 0.05) versus the values obtained for the same channels in the absence of DTT.