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Overview of nascent proteomic techniques. (A) pSILAC: Pulsed stable isotope labeling with amino acids in cell culture. (B) BONCAT: Bio-orthogonal non-canonical amino acid tagging. (C) BONLAC: Combination of BONCAT and pSILAC. (D) mMet-BONCAT: Bio-orthogonal non-canonical amino acid tagging with expanded, mutant methionyl-tRNA synthetases. (E) SUnSET: Surface sensing of translation. (F) PUNCH-P: Puromycin-associated nascent chain proteomics.
