Figure 5.

Magnified Raman spectra between 1500 to 1800 cm⁻¹ (A) and change in integrated intensity ratio of the total of two BSA peaks at 1600 and 1653 cm⁻¹ to the OCP peak at 957 cm⁻¹ (B) after incubation with BSA solutions. ^ap < 0.01 significant difference for 0.20, 0.25 and 1.0 mg·mL⁻¹ BSA in Ca3.0P1.0; ^bp < 0.01 significant difference for 0.20, 0.25 and 1.5 mg·mL⁻¹ in Ca1.5P1.0 solution; ^cp < 0.01 significant difference for 0.20, 0.25 and 1.0 mg·mL⁻¹ BSA in Ca3.0P1.0. ^dp < 0.01 significant difference between 0.20 and 0.75 mg·mL⁻¹ BSA, and ^ep < 0.05 significant difference from 1.5 mg·mL⁻¹ BSA in Ca1.5P1.0; ^fp < 0.01 significantly different for 1.0 and 1.5 mg·mL⁻¹ BSA in Ca3.0P1.0; ^gp < 0.05 significant difference for 0.20 mg·mL⁻¹ BSA and ^hp < 0.01 significant difference from 1.0 mg·mL⁻¹ BSA in Ca1.5P1.0. ⁱp < 0.05 significant difference from 1.5 mg·mL⁻¹ BSA in Ca1.5P1.0. Mean values were obtained from three independent experiments (n = 3).