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. 2019 Jul 14;11(7):982. doi: 10.3390/cancers11070982

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Combined treatment with Oxaliplatin and DHA induces endoplasmic reticulum (ER) stress overproduction. (A,B) HCT116 cells were exposed to different doses of Oxaliplatin and DHA (A) and time periods (B). HCT116 cell lysates were analyzed with an immunoblotting assay using ER stress-related antibodies. (C) C/EBP homologous protein (CHOP) was confirmed via immunofluorescence in the Oxaliplatin and DHA combination (Scale Bar, 10 μM). (D,E) Following transfection with control siRNA or CHOP siRNA, the mRNA(D) and protein (E) levels of LC3 and p62 were detected via qRT-PCR and immunoblotting. **, p < 0.001. (F) Cells were exposed to Oxaliplatin and DHA with or without thapsigargin, and the p62 and LC3 levels were observed via western blotting.