Figure 5.

CHOP regulates SESN2 activity by binding directly to the SESN2 promoter region. (A) The correlation of SESN2 expression and ER stress-related genes in CRC patients. (B) HCT116 cells were pre-treated with thapsigargin for 1 h and then exposed to the Oxaliplatin and DHA combination for 24 h. SESN2 protein expression was confirmed with western blotting. (C–F) Following transfection with control siRNA, CHOP siRNA, or SESN2 siRNA, the SESN2 and CHOP protein levels were determined using qRT-PCR (C,E) and immunoblotting (D,F). ***, p < 0.001. (G) Illustration of the three predicted CHOP binding sites (BS) and ATF BS in the SESN2 promoter. (H) Cells were treated with Oxaliplatin and DHA, and then immunoprecipitated with either IgG or CHOP.