Figure 6.

Combinatorial treatment with DHA and Oxaliplatin increases CHOP expression and in vivo autophagy. (A–D) Nude mice were subcutaneously inoculated with 1 × 107 HCT116-Luc+ Cells. When tumor volume reached approximately 200 mm³, the mice were treated with EtOH, DHA (10 mg/kg), Oxaliplatin (10 mg/kg), or the combinatorial treatment of DHA and Oxaliplatin (10 mg/kg), administered 3 times a week for 2 weeks. (A) Mice were imaged using the NightOWL LB983 bioluminescence imaging (BLI) system. (B) Line graph illustrating the tumor volume (mm³). ***, p < 0.001. (C) Tumor tissues were harvested on day 14 and then imaged using a digital camera. (D) Graph illustrating body weight. **, p < 0.01. (E,F) Immunohistochemistry (IHC) showing LC3 (upper panel), CHOP (middle panel), and SESN2 (lower panel) in the tumors from xenograft at a 400× magnification. (Scale bar, 20 μm). **, p < 0.01 and ***, p < 0.001.