Figure 3.

Generation of tumors and recapitulation of original tumor tissues by temporal expression of additional oncogenes in MC type HOV line. (A) Expression of the transgenes and their surrogate markers as well as vinculin as a loading control were detected by western blotting. (B) Cell growth curves of HOV29T/tetOFF/EMR cells with or without DOX. Each point is the mean of the triplicates ± SEM. (C) In vivo tumor generation capability of HOV29T/tetOFF/EMR cells. Cells were subcutaneously injected into nude mice. At day 44 when the average volume of tumors injected at 4 sites per mouse reached 300mm3, three mice were fed with drinking water supplemented with DOX (1 mg/mL) and sacrificed at 4 days (red), 12 days (green) and 22 days (purple) post-administration. Each point is the mean of the tumor volume ± SEM (n = 4). (D) Histopathology of HOV29T/tetOFF/EMR xenograft and original tumor of HOV29T, H&E. Scale bar 100 μm