Figure 1.

Log-transformed median mediator secretion increase in AML cells after incubation in TLR single agonists for 48 h. For each of the 20 soluble mediators (right part of the figure) we estimated and log-transformed the median effect of the four various TLR agonists (see top of the figure), i.e., the figure is based on the median results for all patients for each agonist/soluble mediator combination. Median values were used in order not to over-estimate the change from low basal levels, and at the same time not to under-estimate the change from basal levels that were close to detection limit of the assay. IL-6 was the most readily upregulated cytokine with medians of 5-fold (Pam3CSK4), 10-fold (R848), 45-fold (flagellin) and 300-fold (LPS) concentration increases. The secretion of the protease inhibitors cystatin C and serpin E1 on the other hand were not altered by TLR ligands.