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. 2019 Jul 22;8(7):761. doi: 10.3390/cells8070761

Figure 2.

Figure 2

Double-fluorescent IHC for CXCR4 and CD34 in tumor and nontumor areas. (a) CXCR4 stained in stromal vessels and tumor cells (red). (b) CD34 stained only on vessels in the tumor stroma (green). (c) A merged IHC image of CD34 and CXCR4. Nuclei were stained with DAPI. Arrowheads indicate CXCR4/CD34 double-positive tumor vessels in the OSCC stroma. (d) CXCR4 stained in the nontumor area (red). (e) CD34 stained in the nontumor area (green). (f) A merged IHC image of CD34 and CXCR4. Nuclei were stained with DAPI. CD34-positive endothelial cells were all negative for CXCR4 (arrowheads).