Figure 3.

CXCR4 antagonist AMD3100 induced tumor necrosis. Mice were injected with HSC-2 cells subcutaneously in the back and head. Seven days after tumor transplantation, AMD3100 or saline was intraperitoneally administrated every day for 21 d. (a) A schematic protocol of tumor injection and AMD3100 administration. (b) Representative photographs of subcutaneous tumors formed in mice. Necrosis and bleeding were observed only in the AMD3100 group. (c) Definition of necrotic areas. Top, HE staining. The areas closed with rectangles are enlarged in “d” and “e”. Bottom, diagrams defining necrotic areas in each tumor. (d,e) High-power magnification views of tumor necrotic areas in the saline-treated mouse (d) and AMD3100-treated mouse (e). The areas are enlarged from Figure 3c. (f) Box-whisker-dot plot of tumor necrotic areas with or without AMD3100 administration. * p = 0.0416, N = 5 mice.