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. 2019 Jul 10;8(7):696. doi: 10.3390/cells8070696

Figure 4.

Figure 4

5′ splice-site mutations do not affect SRSF2-mediated 3′AG′ activation. (A) (Left panel) Schematic diagram of the E7-5′cons minigene where the 5′ss has been changed to a conserved sequence. (Right panel) RT-PCR analysis of the E7-5′cons minigene in SRSF2-expressing cells. (B) (Left panel) Schematic diagram of the E6-5′cons minigene including the conserved 5′ss sequence of exon 6. (Right panel) RT-PCR analysis of the E6-5′cons minigene in SRSF2-expressing cells. (C) (Left panel) Schematic diagram of the E7-ss-mut minigene where the splice signals of exon 7 have been abolished. (Right panel) RT-PCR analysis of the E7-ss-mut minigene in SRSF2-expressing cells.