Table 1.
Summary of Dyes Performance for STED Imaging in Bacterial Cells.
| Nanobody-Dye | Target | Signal-to-Background Ratio | Cellular Background |
|---|---|---|---|
| NBGFP-Atto647n | GFP | 12 | 11 % |
| NBRFP-Atto647n | mCherry2 | 3 | 36 % |
| NBGFP-Atto594 | GFP | n.a. * | n.a. * |
| NBRFP-Atto594 | mCherry2 | 3 | 38 % |
| NBRFP-Star600 | PAmCherry | 2.4 | 42 % |
| NBGFP-Star635p | GFP | 3 | 34 % |
All targets were fused to DivIVA protein. Signal-to-cellular background ratio and cellular background were calculated as described in the main text and in Figure S4. * not applicable (n.a.), values corresponding to signal and cellular background could not be determined due to high cellular background.