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. 2019 Jul 11;20(14):3409. doi: 10.3390/ijms20143409

Figure 3.

Figure 3

AUR protects against rotenone-induced ROS production. (A–D) SN4741 cells were incubated with rotenone (Rot) for 6 h, with or without AUR pretreatment for 1 h. Cells were stained with DCFDA or MitoSOX™, and fluorescence intensity was measured by flow cytometry. Total ROS was determined by measuring DCFDA-stained cells (A,B), and mitochondrial ROS was determined by measuring MitoSOX™-stained cells (C,D). Median fluorescence intensity (MIF) values are presented as means ± SD of three experiments (* P < 0.05, ** P < 0.01 vs. corresponding controls). CN, control.