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. 2019 Jul 19;20(14):3535. doi: 10.3390/ijms20143535

Figure 3.

Figure 3

DRAK1 overexpression activates JNK signaling and caspase 3 cleavage to promote apoptosis. (A) PC3 cells were transfected with DRAK1 and cell lysates from the indicated time points were immunoblotted with indicated antibodies. PC3 cells were treated with JNK inhibitor (SP600125, 25 μM) or caspase 3 inhibitor (zVAD-FMK, 20 μM) and transfected with DRAK1 for 24 h and (B) Cell lysates were probed with indicated antibodies and normalized to β-actin levels, (C) Cell viability analyzed by MTT assay. (D) PC3 cells were transfected with vector alone or DRAK1 in the presence or absence of caspase 3 inhibitor (zVAD-FMK, 20 μM). Caspase 3/7 activity was measured in cell lysates after 24 h. (E) Cells expressing DRAK1 or vector alone were stained with TMRE at indicated times and fluorescence intensity was normalized to control cells. Values shown are mean ± s.d of triplicate assays. * p < 0.01, ** p < 0.001, *** p < 0.0001, n.s: not significant.