Table 2.
Tissue reactions by histometric scoring used to grade inflammation, fibrosis, necrosis, and neovascularization in the tissue surrounding subcutaneous implants.
| Nanofibers | Explan Tation (days) | Edema | PMN | MONO LYM | MO | GC | PC | EOS | FC | NV |
|---|---|---|---|---|---|---|---|---|---|---|
| Control mice | 1 | − | + | − | − | − | − | − | − | − |
| 7 | − | − | + | + | − | − | − | − | − | |
| PET_10_ctrl | 1 | ++++ | ++++ | +++ | ++ | + | − | + | + | − |
| 7 | +++ | ++ | ++++ | ++++ | ++ | ++ | + | ++++ | − | |
| PET_7.5_ctrl | 1 | +++ | +++ | + | + | + | + | + | + | − |
| 7 | ++ | + | ++ | +++ | ++ | + | + | +++ | − | |
| PET_5_ctrl | 1 | + | +++ | sp | + | sp | − | + | + | − |
| 7 | ++ | + | ++ | +++ | + | + | + | ++ | − | |
| PET_2.5_ctrl | 1 | + | +++ | sp | + | sp | − | + | + | − |
| 7 | ++ | + | ++ | +++ | + | + | + | ++ | + | |
| PET_10_NanoAg | 1 | − | +++ | ++ | ++ | − | − | + | + | − |
| 7 | − | + | +++ | +++ | − | + | + | +++ | + | |
| PET_7.5_NanoAg | 1 | − | ++ | sp | sp | − | + | + | + | − |
| 7 | − | + | ++ | ++ | − | + | + | +++ | + | |
| PET_5_NanoAg | 1 | − | ++ | sp | + | − | − | + | + | − |
| 7 | − | + | + | +++ | − | + | + | ++ | + | |
| PET_2.5_NanoAg | 1 | − | ++ | sp | + | − | − | + | + | − |
| 7 | − | + | + | + | − | + | sp | + | ++ |
PMN: polymorphonuclear neutrophils; MONO: monocytes; LYM: lymphocytes; EOS: eosinophils; MO: macrophages; PC: plasma cell; GC: giant cell; FC: fibrocytes, NV: neovascularization. Tissue reactions are rated from − (not present), and sp (sporadic) to ++++ (extensive).