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. 2019 Jun 28;11(7):903. doi: 10.3390/cancers11070903

Figure 2.

Figure 2

Gene expression of single cells and pools of isolated cell line cells. (A) Dendrogram and heat map analyses of Cq measurements of single MCF-7, MCF-7/TAMR1, and MDA-MB-231 cells (n = 2 per cell line) and pools of five cells (n = 1). Each gene is measured in triplicate. Data are mean centered, with mean expression responses to zero. Yellow indicates high expression and blue color represents downregulation relative to the mean of the pool. (B) Bar graph showing significant differences in transcript expression between MCF-7 and MDA-MB-231 cells (*, p < 0.05, ** p < 0.01, *** p < 0.001). (C) Bar graph showing significant differences in transcript expression between MCF-7 and MCF-7/TAMR1 cells (*** p < 0.001).