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. 2019 Jul 11;8(7):704. doi: 10.3390/cells8070704

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence staining to study effects of pH_o on primary cilia. (a) Cells (wild-type and Tg737) were stained with ciliary marker (acetylated-α-tubulin; green) and nucleus marker (DAPI; blue). Representative images are shown for wild-type cells at different pH_o and Tg737 at pH_o 7.4. White boxes show enlargement of the images to depict the presence of primary cilia. (b) The lengths of primary cilia from 50 cells were measured from each preparation (N = 3) and illustrated in the bar graph to depict length distribution within each pH_o. (c) Cilia length was averaged from 150 cells (N = 3; each with 50 randomly selected cells) and the number of cells possessing cilia represented as a percentage with each point representing an individual experimental datapoint. * indicates a significant difference to control pH_o 7.4.

Immunofluorescence staining to study effects of pH_o on primary cilia. (a) Cells (wild-type and Tg737) were stained with ciliary marker (acetylated-α-tubulin; green) and nucleus marker (DAPI; blue). Representative images are shown for wild-type cells at different pH_o and Tg737 at pH_o 7.4. White boxes show enlargement of the images to depict the presence of primary cilia. (b) The lengths of primary cilia from 50 cells were measured from each preparation (N = 3) and illustrated in the bar graph to depict length distribution within each pH_o. (c) Cilia length was averaged from 150 cells (N = 3; each with 50 randomly selected cells) and the number of cells possessing cilia represented as a percentage with each point representing an individual experimental datapoint. * indicates a significant difference to control pH_o 7.4.