Figure 3.

Decreasing extracellular pH increases ERK1/2 phosphorylation in wild-type and Tg737 cells. (a) Representative immunoblots show ERK1/2 phosphorylation in wild-type and Tg737 cells as they are exposed to media of decreasing extracellular pH (pH_o) from 7.4 to 5.5. (b) Bar graph shows the mean p-ERK/ERK, where wild-type cells phosphorylate ERK1/2 at pH of 6.5 while the same level of phosphorylation occurs at a lower pH of 6.0. * indicates p < 0.05 as compared to control pH_o 7.4; N = 10 for wild-type cells and N = 10 for Tg737 cells.