Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 14;20(14):3456. doi: 10.3390/ijms20143456

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The schematic representation of collecting human skeletal muscle cells obtained from extra tissues containing orbicularis oculi muscles at the time of ophthalmic surgery. (a) Surgically excised eyelid tissues soaked in cold PBS solution (left panel), an example of the actual size of the extra eyelid tissue compared with a 1.5-mL microtube (middle panel), and the obtained tissue finely chopped by scissors (right panel). (b) Morphological features of isolated tissues, mass of lipids (arrowheads in left panel), blood capillaries (arrowheads in middle panel), and disconnected skeletal muscle fibers (arrowheads in right panel). Scale bars, 200 μm. (c) Chopped samples were enzymatically treated with collagenase type 2 (left panel), then filtrated after enzymatic digestion (middle panel), and centrifuged to collect single myogenic cells (arrow, right panel).