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. 2019 Jul 14;20(14):3456. doi: 10.3390/ijms20143456

Figure 3.

Figure 3

CD56+CD82+ double-positive myogenic cells from primary cultured orbicularis oculi muscles. (a) Image of primary cultured cells digested from isolated eyelid tissue. (b) FACS profile for detecting CD56-positive cells from primary cultured cells with extra eyelid tissue. (c) Morphological features of CD56-sorted cells (passage 1, p1) of orbicularis oculi muscles, cultured for 24 h (24hrs), 48 h (48hrs), and 7 days (day7). (d) Myogenic differentiation with CD56+ cells in 2% horse serum medium for 7 days (DM, day7). MF20, green; DAPI, blue. (e) The percentage of differentiated myogenic cells is presented as MF20/DAPI ratio. n = 3 independent replicates; p-values were determined by t-test from a two-tailed distribution. * p < 0.01. (f) FACS profile of CD56- and CD82- double positive cells from primary cultured orbicularis oculi muscles from extra eyelid tissue. (g) PAX7 transcriptome analyses of CD56+CD82+ sorted cells shown in (f). n = 3 independent replicates; p-values are determined by Dunnett’s multiple-comparisons test. * p < 0.01. All scale bars, 100 μm.