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. 2019 Jul 16;20(14):3482. doi: 10.3390/ijms20143482

Figure 2.

Figure 2

Molecular analysis of tobacco transgenic plants. (a,b) Primary transformant plants were analyzed for the presence of SmchiC and nptII genes by PCR amplification. Lane 1–12; transgenic tobacco lines, (-) water amplification control; (+) positive control (p2x35SChiC) vector; WT wild-type plant (negative control); M, molecular markers. (c,d) chitinase and actin expression by RT-PCR in homozygous transgenic plants. Transformed tobacco lines, namely lines 3.1, 5.5, 9.5 and 12.4; wt wild-type plant (negative control); M, molecular marker.