Figure 5.

The effect of chitinase SmChiC protein in leaf extracts from homozygous transgenic tobacco plants over B. cinerea growth and spore germination. (a) After 48 h of incubation, percentage of fungal growth inhibition is defined as 100× the ratio of the corrected A₅₉₅ of the control plant minus the corrected A₅₉₅ of the sample over the corrected A₅₉₅ of the control plant. Each bar value represents the mean ± SD of triplicate experiments (Student’s t test; ***, p = 0.0002; *, 0.0165; **, 0.0053 for each line respectively versus control). (b) Microscopic analyses of the appearance of the B. cinerea hyphae after incubation for 48 h in presence of different plant crude extract. Magnification target (40×). (c) Microscopic analyses of the appearance of B. cinerea single spore hyphae after incubation for 48 h in presence of different plant crude extract. Magnification 40×.